Part:BBa_K1402016
Ara-RBS-BTE-Treminator
Acyl carrier protein (ACP) thioesterase catalyzes the hydrolysis of acyl-ACP into fatty acid and ACP. This reaction removes the coupling between fatty acid biosynthesis and phospholipid biosyntheis, which is a key to obtaining free fatty acids. This thioesterase BTE can produce fatty acids with 12~14 carbon chain length. In previous reserches, thioesterase gene BTE can be expressed in E.coli BL21(DE3) and enhance the synthesis of free fatty acids.The RBS and ara promoter are before the BTE coding sequence and T7 terminator is after BTE. Ara promoter is a direct copy of Pbad promoter in E.Coli DH5α,the natural promoter for the Ara operon.We can activate the expression of BTE by adding arabinose in the culture in the absence of glucose.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 797
Illegal BglII site found at 937
Illegal BamHI site found at 236
Illegal BamHI site found at 386 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 71
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1346
Illegal SapI site found at 53
| None |